Part:BBa_K3747128

Nitrite reductase K (nirK) from Pseudomonas stutzeri JM300

This gene is part of the nir operon from P. stutzeri JM300. Nir is the second enzyme of the denitrification pathway, converting nitrite (NO₂^-) into nitric oxide (NO). NirK nitrite reductases are copper-dependent [1]. It has long been thought the presence of NirS (a heme-dependent Nir) and NirK was mutually exclusive, however, this is not true as for example Pseudomonas stutzeri possesses both [2]. In contrast to NirS, NirK does not need any other genes for its maturation or for it to be functionally active. Nevertheless, in some organisms, e.g. P. stutzeri JM300, nirK gene is accompanied by nirV gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 487
  Illegal NgoMIV site found at 541
  Illegal NgoMIV site found at 907
  Illegal NgoMIV site found at 991
  Illegal AgeI site found at 352
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] L. Wittorf, C.M. Jones, G. Bonilla-Rosso, S. Hallin, Research in Microbiology Expression of nirK and nirS genes in two strains of Pseudomonas stutzeri harbouring both types of NO-forming nitrite reductases, Res. Microbiol. 169 (2018) 343–347. https://doi.org/10.1016/j.resmic.2018.04.010.

[2] D. Helen, H. Kim, B. Tytgat, W. Anne, Highly diverse nirK genes comprise two major clades that harbour ammonium- producing denitrifiers, BMC Genomics. 17 (2016) 1–13. https://doi.org/10.1186/s12864-016-2465-0.